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<PublisherName>developer</PublisherName>
<JournalTitle>International journal of Information Technology</JournalTitle>
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<EISSN>n</EISSN>
<Volume-Issue>Volume 38, Number 1</Volume-Issue>
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<IssueTopic>Multidisciplinary</IssueTopic>
<IssueLanguage>English</IssueLanguage>
<Season>Jan-March</Season>
<SpecialIssue>N</SpecialIssue>
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<Month>11</Month>
<Day>30</Day>
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<ArticleTitle>PHYTOCHEMICALS CONSTITUENT ANALYSIS AND CELL CYCLE INHIBITION EFFECT OF ETHANOL EXTRACT OF Litsea cubeba Lour. HEARTWOOD TOWARDS MCF-7/HER-2 CELL LINE</ArticleTitle>
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<ArticleLanguage>English</ArticleLanguage>
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<FirstPage>100</FirstPage>
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<Author>
<FirstName>A.</FirstName>
<LastName>Dalimunthe1</LastName>
<AuthorLanguage>English</AuthorLanguage>
<Affiliation/>
<CorrespondingAuthor>N</CorrespondingAuthor>
<ORCID/>
<FirstName>P.Sitorus2 and D.</FirstName>
<LastName>Satria2</LastName>
<AuthorLanguage>English</AuthorLanguage>
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<Abstract>Attarasa (Litsea cubeba Lour.) heartwood is potential anticancer especially for breast cancer which is overexpressed in HER-2. This study evaluated phytochemicals analysis of ethanol extract, cytotoxicity, cell cycle inhibition and p53 expression of ethanol extract (EE) of Litsea cubeba Lour. heartwood. EE was analyzed for phytochemicals content with LC-HRMS, cytotoxicity activity was determined by MTT method towards breast cancer cell line MCF-7/Her-2. Cell cycle inhibition and p53 expression were analyzed with the flow cytometry method. EE was found to contain 24 compounds. EE was found to have IC50 191.63 ---PlusMinusSymbol--- 1.56 µg/mL, at concentration 40 µg/mL caused accumulation in G2- M phase (17.71% to 23.34%) and increased p53 expression (0.99% to 17.63%). The results utter that EE of Litsea cubeba Lour. heartwood has a cytotoxic effect through inhibition of the cell cycle. Our further study is to assess the molecular mechanism that responsible for anticancer activity.</Abstract>
<AbstractLanguage>English</AbstractLanguage>
<Keywords>Breast Cancer, MCF-7/Her-2, Litsea cubeba Lour., Heartwood, Ethanol Extract.</Keywords>
<URLs>
<Abstract>https://developer.ubipayroll.com/ubijournal-v1copy/journals/abstract.php?article_id=13118&title=PHYTOCHEMICALS CONSTITUENT ANALYSIS AND CELL CYCLE INHIBITION EFFECT OF ETHANOL EXTRACT OF Litsea cubeba Lour. HEARTWOOD TOWARDS MCF-7/HER-2 CELL LINE</Abstract>
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<References>
<ReferencesarticleTitle>References</ReferencesarticleTitle>
<ReferencesfirstPage>16</ReferencesfirstPage>
<ReferenceslastPage>19</ReferenceslastPage>
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