HYDROCHALCONE COMPOUNDS FROM INDONESIAN MEDICINAL PLANT, “SIRIH HUTAN”, Piper aduncum

Author: R. Kartika1, *, Sudrajat1 , Bustanussalam2 and P. Simanjuntak2, 3

Category: Medicinal Article

Abstract:

Isolation, purification and identification of two hydrochalcone compounds from Indonesian medicinal plant “Sirihhutan”, Piper aduncum (Piperaceae) had been done. Isolation and purification of ethylacetate extract subjected to column chromatography (SiO2; (i).n-hexane-ethylacetate = 10:1 ~ 1 : 1; ethylacetate (ii).n-hexaneethylacetate = 8: 1 gave two pure compounds. Based on infra red, 1D __ampersandsign 2D-NMR, mass spectral data and comparison chemical shift of protons and carbons, the isolated compounds are 2’,6’-dihydroxy-4’-methoxy dihydrochalcone and 2’,6’,4-trihydroxy-4’-methoxy dihydrochalcone with free radical scavenging inhibitionvalues are 21.77 % and 90.1 % respectively.

Keywords: Hydrochalcone; Piper aduncum; Piperaceae; 2’, 6’-dihydroxy-4’-methoxy dihydrochalcone; 2’, 6’, 4- trihydroxy-4’-methoxy dihydrochalcone

Full Text:

This research is a continuation of chemical studies on Indonesian medicinal plants, especially on bioactive chemical compounds as antioxidant.1-5 This research focused on hydrochalcone compounds from“SirihHutan” (localname), Piper aduncum L.(Piperaceae). Traditionally, this plant isused as a deodorant, antihaemorraghea, antivomit, anti-septic, antibacterial and antifungi6 . Based on the literature, this plant has activities as antibacteria6 , insectiside7 , andantioxidant8 . Isolation results of antioxidant bioactive compounds from ethylacetate fraction of P.aduncum L based on free radicals cavenging activity test usingDPPH (1,1-diphenylpycrilhidrazine) gave two hydrochalcone compounds, i.e.2’,6’-dihydroxy-4’-methoxy dihydrochalcone and 2’,6’,4-trihydroxy-4’-methoxy dihydrochalcone.

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